SEAVIEW and PHYLO_WIN: Two Graphic Tools for Sequence Alignment and Molecular Phylogeny

Comput Appl Biosci. 1996 Dec;12(6):543-8. doi: 10.1093/bioinformatics/12.6.543.


SEAVIEW and PHYLO_WIN are two graphic tools for X Windows-Unix computers dedicated to sequence alignment and molecular phylogenetics. SEAVIEW is a sequence alignment editor allowing manual or automatic alignment through an interface with CLUSTALW program. Alignment of large sequences with extensive length differences is made easier by a dot-plot-based routine. The PHYLO_WIN program allows phylogenetic tree building according to most usual methods (neighbor joining with numerous distance estimates, maximum parsimony, maximum likelihood), and a bootstrap analysis with any of them. Reconstructed trees can be drawn, edited, printed, stored, evaluated according to numerous criteria. Taxonomic species groups and sets of conserved regions can be defined by mouse and stored into sequence files, thus avoiding multiple data files. Both tools are entirely mouse driven. On-line help makes them easy to use. They are freely available by anonymous ftp at mol_phylogeny or, or by e-mail to

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Computer Graphics*
  • DNA / genetics
  • Evolution, Molecular
  • Molecular Sequence Data
  • Phylogeny*
  • Proteins / genetics
  • RNA, Ribosomal / genetics
  • Sequence Alignment / methods*
  • Sequence Alignment / statistics & numerical data
  • Software


  • Proteins
  • RNA, Ribosomal
  • DNA