Intrahepatic expression of pro-inflammatory cytokine mRNAs and interferon efficacy in chronic hepatitis C

Liver. 1996 Dec;16(6):390-9. doi: 10.1111/j.1600-0676.1996.tb00768.x.

Abstract

To investigate the relationship between intrahepatic cytokine expression and interferon (IFN) response in chronic hepatitis C [CH(C)], interleukin (IL)-1 beta, -2, -4, -6, -8, interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha and TNF-beta mRNAs were investigated semiquantitatively by reverse transcription polymerase chain reaction using serial liver biopsies taken before and after IFN-alpha treatment from 24 patients with CH(C), including 12 responders and 12 non-responders. Before IFN treatment, IL-2, TNF-beta, IFN-gamma and IL-8 mRNA were associated with severe hepatitis activity whereas IL-4 mRNA was associated with weak hepatitis activity, regardless of IFN response. IL-2, TNF-beta and IFN-gamma mRNAs were significantly greater in IFN non-responders. After IFN treatment a complete response to IFN was significantly associated with the disappearance of these pro-inflammatory cytokines, whereas non-responders retained the expression of cytokine mRNA as before IFN treatment. Our results indicated that IFN-alpha treatment may modulate the intrahepatic cytokine network, and this may be one mechanism of IFN-alpha that reduces hepatitis activity, aside from an anti-viral effect. A difference in cytokine network may be involved in IFN response in CH(C).

MeSH terms

  • Adult
  • Chronic Disease
  • Cytokines / biosynthesis*
  • Female
  • Hepatitis C / drug therapy
  • Hepatitis C / metabolism*
  • Humans
  • Interferon alpha-2
  • Interferon-alpha / therapeutic use*
  • Interferon-gamma / metabolism*
  • Liver / metabolism*
  • Liver / pathology
  • Liver / virology
  • Male
  • Middle Aged
  • RNA, Messenger / biosynthesis*
  • Recombinant Proteins

Substances

  • Cytokines
  • Interferon alpha-2
  • Interferon-alpha
  • RNA, Messenger
  • Recombinant Proteins
  • Interferon-gamma