Neutrophil margination, sequestration, and emigration in the lungs of L-selectin-deficient mice

J Clin Invest. 1997 Feb 1;99(3):526-33. doi: 10.1172/JCI119189.


These studies tested the hypothesis that L-selectin plays a role in neutrophil traffic in the lungs, particularly in neutrophil margination, sequestration, and emigration, using L-selectin-deficient mice. No defect in neutrophil margination within either capillaries or arterioles and venules was observed in uninflamed lungs of L-selectin-deficient mice. The initial rapid sequestration of neutrophils within the pulmonary capillaries 1 min after intravascular injection of complement fragments was not prevented. In contrast, L-selectin did contribute to the prolonged neutrophil sequestration (> or = 5 min). Interestingly, neutrophil accumulation within noncapillary microvessels required L-selectin at both 1 and 5 min after complement injection. During bacterial pneumonias, L-selectin played a role in neutrophil accumulation within noncapillary microvessels in response to either Escherichia coli or Streptococcus pneumoniae and within capillaries in response to E. coli but not S. pneumoniae. However, L-selectin was not required for emigration of neutrophils or edema in response to either organism. These studies demonstrate a role for L-selectin in the prolonged sequestration of neutrophils in response to intravascular complement fragments, in the intracapillary accumulation of neutrophils during E. coli-induced pneumonia, and in the accumulation of neutrophils within noncapillary microvessels when induced by either intravascular complement fragments or

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Arterioles / immunology
  • Capillaries / immunology
  • Cell Adhesion / genetics*
  • Cell Adhesion / immunology*
  • Cell Movement / genetics*
  • Cell Movement / immunology*
  • Complement System Proteins / pharmacology
  • Edema / immunology
  • Escherichia coli
  • Inflammation / genetics*
  • L-Selectin / genetics*
  • Leukocyte Count
  • Lung / immunology*
  • Mice
  • Mice, Mutant Strains
  • Mutagenesis*
  • Neutrophils / immunology*
  • Neutrophils / physiology*
  • Pneumonia, Bacterial / immunology
  • Streptococcus pneumoniae
  • Venules / immunology


  • L-Selectin
  • Complement System Proteins