Production and Characterization of a New Monoclonal Antibody Effective in Recognizing the CD3 T-cell Associated Antigen in Formalin-Fixed Embedded Tissue

Histopathology. 1997 Jan;30(1):16-22. doi: 10.1046/j.1365-2559.1997.d01-553.x.

Abstract

Phenotypic analysis of lymphoproliferative disorders is now considered mandatory for accurate classification which is the basis for optimum patient management. This is presently carried out in most cases using a range of antibodies recognizing B and T-cell antigens effective in paraffin sections, and an antibody to CD 3 is currently a key member of such panels, indicating T-cell phenotype. Current antibodies to CD3 are polyclonal with the inherent disadvantages of this type of reagent compared to monoclonal antibodies. In this study, we have used a recombinant fusion protein representing part of the epsilon subunit of the CD3 molecule to generate a novel monoclonal antibody (NCL-CD3-PS1) effective in paraffin sections. The antibody has been characterized biochemically and by immunohistochemistry using a wide range of normal and pathological tissues. Lineage and phenotype specificity have been supported in our study and results from other laboratories are awaited with interest.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / biosynthesis*
  • Antibodies, Monoclonal / chemistry*
  • CD3 Complex / analysis
  • CD3 Complex / immunology*
  • Formaldehyde
  • Humans
  • Immunohistochemistry
  • Lymphoma, B-Cell / pathology
  • Lymphoma, T-Cell / pathology
  • Paraffin Embedding
  • Recombinant Proteins / analysis
  • Recombinant Proteins / immunology
  • T-Lymphocytes / chemistry*
  • Tissue Fixation

Substances

  • Antibodies, Monoclonal
  • CD3 Complex
  • Recombinant Proteins
  • Formaldehyde