Functional impairment of rat enterochromaffin-like cells by interleukin 1 beta

Gastroenterology. 1997 Feb;112(2):364-75. doi: 10.1053/gast.1997.v112.pm9024290.


Background & aims: Histamine-producing enterochromaffin-like (ECL) cells play an integrative role in the regulation of acid secretion. Decreased mucosal histamine concentrations and increased levels of interleukin (IL) 1 beta, IL-6, and IL-8 have been detected in the gastric mucosa inflamed with Helicobacter pylori. The aim of this study was to investigate the response of isolated ECL cells to these cytokines.

Methods: Enriched rat gastric ECL cells (85%-95%) were cultured for 2-4 days.

Results: Polymerase chain reaction showed IL-1 and IL-6, but not IL-8 receptors, in ECL cell complementary DNA. Positive receptor staining with biotinylated IL-1 beta corresponded to ECL cell enrichment (92%). IL-6 and IL-8 had no effect on histamine secretion. IL-1 beta (2 U/mL) stimulated basal histamine secretion and nitric oxide production within 60 minutes and cyclic guanosine monophosphate production within 20 minutes. Pretreatment for 20 minutes with IL-1 beta (2 U/mL) attenuated gastrin-stimulated histamine secretion by 40%-50%, reversed by the IL-1 receptor antagonist (10 U/ mL). Pretreatment for 20 minutes with IL-1 beta (2 U/mL) completely inhibited gastrin-stimulated (1 nmol/L) histidine decarboxylase activity. IL-1 beta (2 U/mL, 60 minutes) increased lactate dehydrogenase release to 25% of cell content. Cells pretreated with IL-1 beta did not respond to gastrin after a further 48-hour culture and showed decreased histamine content.

Conclusions: ECL cells appear to express IL-1 receptors. IL-1 beta causes sustained functional impairment of ECL cells in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biotin
  • Cyclic GMP / biosynthesis
  • DNA, Complementary / genetics
  • Dinoprostone / pharmacology
  • Enterochromaffin Cells / cytology
  • Enterochromaffin Cells / drug effects*
  • Enterochromaffin Cells / physiology*
  • Epinephrine / pharmacology
  • Female
  • Gastrins / pharmacology
  • Gene Library
  • Histamine Release / drug effects
  • Histidine Decarboxylase / metabolism
  • Interleukin-1 / pharmacology*
  • Interleukins / pharmacology
  • L-Lactate Dehydrogenase / metabolism
  • Nitric Oxide / biosynthesis
  • Polymerase Chain Reaction
  • Rats
  • Rats, Sprague-Dawley
  • Time Factors


  • DNA, Complementary
  • Gastrins
  • Interleukin-1
  • Interleukins
  • Nitric Oxide
  • Biotin
  • L-Lactate Dehydrogenase
  • Histidine Decarboxylase
  • Cyclic GMP
  • Dinoprostone
  • Epinephrine