Rapid assay for detection of methicillin-resistant Staphylococcus aureus using multiplex PCR

Mol Cell Probes. 1996 Dec;10(6):471-5. doi: 10.1006/mcpr.1996.0066.


The presence or absence of the mecA gene, the determinant of resistance to all beta-lactam antibiotics, was examined in clinical isolates of Staphylococcus aureus by multiplex polymerase chain reaction (MPCR). Two pairs of primers were used, which yielded two specific products; a 280-bp nuc- based PCR fragment (amplification product of the nuc gene encoding specific Staphylococcus aureus nuclease) and a 533-bp mecA-based PCR fragment (amplification product of the mecA gene). The MPCR system was designed to be incorporated into the work flow in clinical diagnostic laboratories as a routine analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Genes, Bacterial
  • Humans
  • Methicillin Resistance
  • Microbial Sensitivity Tests
  • Polymerase Chain Reaction / methods*
  • Staphylococcus aureus / drug effects*
  • Staphylococcus aureus / isolation & purification