Postsynaptic element contributes to the delay in synaptogenesis in synapsin I-deficient neurons

Mol Cell Neurosci. 1996;8(4):286-99. doi: 10.1006/mcne.1996.0064.


In a previous study, we reported a retardation in process outgrowth and synapse formation in cultured hippocampal neurons from synapsin I-deficient mice. Here we investigated whether this delay in synaptogenesis was attributable to pre- or postsynaptic elements. The experimental paradigm used in this study involved the establishment of heterochronic cocultures of neurons from wild-type and synapsin I-deficient neurons established synapses with mature wild-type postsynaptic elements after 24 and 72 h, respectively. In contrast, synapsin I-deficient postsynaptic elements were able to receive synapses only after 9 days in culture, representing a 5-day delay compared to controls. The results suggest a broad role of synapsin I in the structural development of the synapse, participating directly or indirectly in the maturation of both presynaptic and postsynaptic sites.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Axons / physiology
  • Cell Count
  • Cells, Cultured
  • Dendrites / physiology
  • Dendrites / ultrastructure
  • Hippocampus / cytology
  • Hippocampus / embryology
  • Hippocampus / ultrastructure
  • Mice / embryology
  • Neurons / metabolism*
  • Neurons / physiology*
  • Neurons / ultrastructure
  • Synapses / physiology*
  • Synapsins / deficiency*
  • Time Factors


  • Synapsins