The MIC1 microneme protein of Toxoplasma gondii contains a duplicated receptor-like domain and binds to host cell surface

Mol Biochem Parasitol. 1996 Dec 20;83(2):201-10. doi: 10.1016/s0166-6851(96)02773-9.

Abstract

The cDNA encoding the Toxoplasma gondii microneme protein MIC1 and the corresponding gene have been cloned and sequenced. The MIC1 gene contains three introns. The cDNA encodes a 456 amino acid (aa) sequence, with a typical signal sequence and no other trans-membrane domain. The protein contains a tandemly duplicated domain with conservation of cysteines and presents distant homology with the Plasmodium sp. microneme protein TRAP-SSP2. The MIC1 protein from tachyzoite lysates and a PMAL recombinant expressing the N-terminal duplicated domain of the protein bound to the surface of putative host cells, suggesting a possible involvement of MIC1 in host cell binding/recognition.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Adhesion
  • Cell Adhesion Molecules / chemistry
  • Cell Adhesion Molecules / genetics*
  • Cell Adhesion Molecules / metabolism*
  • Chlorocebus aethiops
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Genes, Protozoan / genetics
  • Introns / genetics
  • Molecular Sequence Data
  • Molecular Weight
  • Protein Binding
  • Protozoan Proteins*
  • Recombinant Fusion Proteins / metabolism
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Toxoplasma / genetics*
  • Toxoplasma / metabolism
  • Vero Cells

Substances

  • Cell Adhesion Molecules
  • DNA, Complementary
  • MIC1 protein, Toxoplasma gondii
  • Protozoan Proteins
  • Recombinant Fusion Proteins

Associated data

  • GENBANK/U42213
  • GENBANK/Z71786