Abstract
The cDNA encoding the Toxoplasma gondii microneme protein MIC1 and the corresponding gene have been cloned and sequenced. The MIC1 gene contains three introns. The cDNA encodes a 456 amino acid (aa) sequence, with a typical signal sequence and no other trans-membrane domain. The protein contains a tandemly duplicated domain with conservation of cysteines and presents distant homology with the Plasmodium sp. microneme protein TRAP-SSP2. The MIC1 protein from tachyzoite lysates and a PMAL recombinant expressing the N-terminal duplicated domain of the protein bound to the surface of putative host cells, suggesting a possible involvement of MIC1 in host cell binding/recognition.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Adhesion
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Cell Adhesion Molecules / chemistry
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Cell Adhesion Molecules / genetics*
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Cell Adhesion Molecules / metabolism*
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Chlorocebus aethiops
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Cloning, Molecular
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DNA, Complementary / genetics
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Genes, Protozoan / genetics
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Introns / genetics
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Molecular Sequence Data
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Molecular Weight
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Protein Binding
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Protozoan Proteins*
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Recombinant Fusion Proteins / metabolism
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Toxoplasma / genetics*
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Toxoplasma / metabolism
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Vero Cells
Substances
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Cell Adhesion Molecules
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DNA, Complementary
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MIC1 protein, Toxoplasma gondii
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Protozoan Proteins
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Recombinant Fusion Proteins
Associated data
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GENBANK/U42213
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GENBANK/Z71786