Polymorphism at the TNF loci in rheumatoid arthritis

Tissue Antigens. 1997 Jan;49(1):74-8. doi: 10.1111/j.1399-0039.1997.tb02715.x.


The purpose of this work was to analyze the possible influence of TNF loci polymorphism on the susceptibility and/or the disease profile of rheumatoid arthritis (RA). Tumor necrosis factor (alpha and beta) genotypes were determined in 60 patients with RA and 102 healthy subjects by a method based on PCR-RFLP with amplification-created restriction sites. The results obtained in the present study showed that there is not a significant association of either TNF alpha promoter variation (at positions -308 and -238) or TNF beta polymorphism with susceptibility to RA. However, a significant difference in the mean age at disease onset was found between -238 TNF alpha genotypes. In addition, a difference in the presence of nodular disease was observed between -308 TNF alpha genotype. The results of this study suggests that the TNF alpha gene may play a role in the disease profile of rheumatoid arthritis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Arthritis, Rheumatoid / genetics*
  • Arthritis, Rheumatoid / immunology
  • Chromosome Mapping
  • Disease Susceptibility
  • HLA-DR Antigens / genetics
  • HLA-DRB1 Chains
  • Humans
  • Lymphotoxin-alpha / genetics*
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*
  • Polymorphism, Restriction Fragment Length
  • Tumor Necrosis Factor-alpha / genetics*


  • HLA-DR Antigens
  • HLA-DRB1 Chains
  • Lymphotoxin-alpha
  • Tumor Necrosis Factor-alpha