Micro-assay method for evaluating the allergenicity of the major soybean allergen, Gly m Bd 30K, with mouse antiserum and RBL-2H3 cells

Biosci Biotechnol Biochem. 1997 Jan;61(1):19-23. doi: 10.1271/bbb.61.19.

Abstract

A micro-assay method for evaluating the allergenicity of soybean allergen was developed by using the mouse antiserum against Gly m Bd 30K, a major soybean allergen, and RBL-2H3, a rat mucosal mast cell line. The antiserum against Gly m Bd 30K was prepared by subcutaneously immunizing BALB/c mice with the allergen. The behavior by affinity-chromatography and the properties against heat treatment show that the reaginic activity of the antiserum resided in the IgE antibody specific for Gly m Bd 30K. The developed assay method is shown to be useful for simulating IgE mediated type-I allergy and to be highly sensitive for detecting the allergen.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens*
  • Animals
  • Antigens, Plant
  • Cells, Cultured
  • Enzyme-Linked Immunosorbent Assay
  • Food Hypersensitivity / diagnosis*
  • Food Hypersensitivity / immunology
  • Hot Temperature
  • Immune Sera / immunology
  • Immune Sera / metabolism*
  • Immune Sera / pharmacology
  • Immunoblotting
  • Immunoglobulin E / metabolism
  • Immunoglobulin G / metabolism
  • Intestinal Mucosa / cytology
  • Intestinal Mucosa / drug effects
  • Intestinal Mucosa / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Plant Proteins / immunology*
  • Plant Proteins / toxicity*
  • Rats
  • Rats, Wistar
  • Soybean Proteins
  • Toxicity Tests / methods
  • beta-N-Acetylhexosaminidases / drug effects
  • beta-N-Acetylhexosaminidases / metabolism

Substances

  • Allergens
  • Antigens, Plant
  • Gly m BD 30K protein, Glycine max
  • Immune Sera
  • Immunoglobulin G
  • Plant Proteins
  • Soybean Proteins
  • Immunoglobulin E
  • beta-N-Acetylhexosaminidases