High-performance liquid chromatographic determination of imidazole dipeptides, histidine, 1-methylhistidine and 3-methylhistidine in equine and camel muscle and individual muscle fibres

J Chromatogr B Biomed Sci Appl. 1997 Jan 10;688(1):47-55. doi: 10.1016/s0378-4347(97)88054-1.

Abstract

The combined solid-phase extraction (Isolute PRS columns) and reversed-phase gradient HPLC method presented provides a sensitive, reproducible and selective quantification of carnosine, balenine, homocarnosine, histidine, 1-methylhistidine and 3-methylhistidine in equine and camel muscle and individual muscle fibres. Recoveries were 91-115%. Lower limits of detection were 0.005-0.010 mmol kg-1 dry muscle. The compounds were isolated from other physiological amino acids and small peptides and resolved within a single chromatographic run of 55 min. Concentrations of these compounds in equine myocardium, diaphragm, skeletal muscle, camel muscle and individual muscle fibres of both species are presented for the first time.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anserine / analysis
  • Camelus / metabolism*
  • Carnosine / analogs & derivatives
  • Carnosine / analysis
  • Chromatography, High Pressure Liquid / methods*
  • Chromatography, High Pressure Liquid / veterinary
  • Circadian Rhythm
  • Dipeptides / analysis*
  • Female
  • Histidine / analogs & derivatives
  • Histidine / analysis*
  • Horses / metabolism*
  • Imidazoles / chemistry*
  • Male
  • Methylhistidines / analysis
  • Muscles / chemistry*
  • Muscles / pathology
  • Osmolar Concentration
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrometry, Fluorescence / veterinary

Substances

  • Dipeptides
  • Imidazoles
  • Methylhistidines
  • N(beta)-alanyl-1-methyl-histidine
  • Histidine
  • Carnosine
  • Anserine