Abstract
The expression of small GTP-binding protein genes of the ras superfamily was examined in pigmented human melanoma cells by a PCR-based strategy. Twenty six different partial cDNA sequences were isolated, including 17 members of the rab subfamily, of which 9 represented novel genes. Some rabs expressed in melanoma cells overlapped with those of platelets: this should prove relevant to the investigation of murine and human disorders characterized by the combination of pigment dilution and a platelet storage pool defect.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blood Platelets / chemistry
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DNA, Complementary
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GTP Phosphohydrolases / analysis
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GTP Phosphohydrolases / genetics*
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GTP-Binding Proteins / analysis
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GTP-Binding Proteins / genetics*
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Gene Expression
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Humans
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Melanoma / chemistry
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Melanoma / genetics*
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Molecular Sequence Data
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Polymerase Chain Reaction
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Tumor Cells, Cultured
Substances
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DNA, Complementary
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GTP Phosphohydrolases
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GTP-Binding Proteins
Associated data
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GENBANK/U66621
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GENBANK/U66622
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GENBANK/U66623
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GENBANK/U66624