The structure of the carbohydrate backbone of the lipopolysaccharide from Acinetobacter strain ATCC 17905

Eur J Biochem. 1997 Jan 15;243(1-2):122-7. doi: 10.1111/j.1432-1033.1997.0122a.x.

Abstract

The structure of the carbohydrate backbone of the lipopolysaccharide from Acinetobacter strain ATCC 17905 was studied. After deacylation of the lipopolysaccharide, a mixture of two compounds (ratio approximately 2:1) was isolated by high-performance anion-exchange chromatography, the structures of which were determined by NMR spectroscopy and electrospray-mass spectrometry as [STRUCUTRE IN TEXT] [Sug, 3-deoxy-D-manno-2-octulopyranosonic acid (Kdo) in oligosaccharide 1 (major portion) and D-glycero-D-talo-2-octulopyranosonic acid (Ko) in oligosaccharide 2 (minor portion)]. All monosaccharide residues also possess the D-configuration and are present in the pyranose form.

MeSH terms

  • Acinetobacter / chemistry*
  • Carbohydrate Sequence
  • Lipopolysaccharides / chemistry*
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Molecular Sequence Data
  • Oligosaccharides / chemistry

Substances

  • Lipopolysaccharides
  • Oligosaccharides