Structural and serological characterisation of the O-specific polysaccharide from lipopolysaccharide of Acinetobacter calcoaceticus strain 7 (DNA group 1)

Eur J Biochem. 1997 Jan 15;243(1-2):167-73. doi: 10.1111/j.1432-1033.1997.0167a.x.


S-form lipopolysaccharide was isolated by phenol/water extraction from a strain of Acinetobacter calcoaceticus (DNA group 1 ). The structure of the O-antigenic polysaccharide was determined by compositional analysis and NMR spectroscopy of the de-O-acylated lipopolysaccharide. The isolated polysaccharide obtained after hydrolysis of lipopolysaccharide in 0.01 M trifluoroacetic acid has the following structure: [STRUCTURE IN TEXT] in which Pyr is pyruvate. The O-acetyl substitution of D-Gal was non-stoichiometric. The O-antigen was specifically recognised in western blots by polyclonal rabbit antisera.

MeSH terms

  • Acinetobacter calcoaceticus / chemistry*
  • Animals
  • Antibodies, Bacterial / immunology
  • Antigens, Bacterial / chemistry*
  • Lipopolysaccharides / chemistry*
  • Magnetic Resonance Spectroscopy
  • Rabbits


  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Lipopolysaccharides