Analysis of four tylosin biosynthetic genes from the tylLM region of the Streptomyces fradiae genome

Gene. 1997 Jan 15;184(2):197-203. doi: 10.1016/s0378-1119(96)00595-1.

Abstract

The tylLM region of the tylosin biosynthetic gene cluster of Streptomyces fradiae contains four open reading frames (orfs1*-4*). The function of the orf1* product is not known. The product of orf2* (tylM2) is the glycosyltransferase that adds mycaminose to the 5-hydroxyl group of tylactone, the polyketide aglycone of tylosin (Ty). A methyltransferase, responsible for 3-N-methylation during mycaminose production, is encoded by orf3* (tylM1). The product of orf4* (cer) is crotonyl-CoA reductase, which converts acetoacetyl-CoA to butyryl-CoA for use as a 4C extender unit during tylactone production.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / biosynthesis*
  • Anti-Bacterial Agents / chemistry
  • Chromosome Mapping
  • Chromosomes, Bacterial
  • DNA, Bacterial
  • Genes, Bacterial
  • Genetic Complementation Test
  • Glucosamine / analogs & derivatives
  • Glucosamine / biosynthesis
  • Molecular Sequence Data
  • Molecular Structure
  • Multigene Family
  • Open Reading Frames
  • Sequence Homology, Amino Acid
  • Streptomyces / enzymology
  • Streptomyces / genetics*
  • Tylosin / biosynthesis*
  • Tylosin / chemistry

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • mycaminose
  • Glucosamine
  • Tylosin

Associated data

  • GENBANK/D13307
  • GENBANK/D13308
  • GENBANK/L28170
  • GENBANK/M74717
  • GENBANK/U10405
  • GENBANK/U37135
  • GENBANK/X06150
  • GENBANK/X63451
  • GENBANK/X81885
  • GENBANK/Z48268