Abstract
The tylLM region of the tylosin biosynthetic gene cluster of Streptomyces fradiae contains four open reading frames (orfs1*-4*). The function of the orf1* product is not known. The product of orf2* (tylM2) is the glycosyltransferase that adds mycaminose to the 5-hydroxyl group of tylactone, the polyketide aglycone of tylosin (Ty). A methyltransferase, responsible for 3-N-methylation during mycaminose production, is encoded by orf3* (tylM1). The product of orf4* (cer) is crotonyl-CoA reductase, which converts acetoacetyl-CoA to butyryl-CoA for use as a 4C extender unit during tylactone production.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / biosynthesis*
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Anti-Bacterial Agents / chemistry
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Chromosome Mapping
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Chromosomes, Bacterial
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DNA, Bacterial
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Genes, Bacterial
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Genetic Complementation Test
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Glucosamine / analogs & derivatives
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Glucosamine / biosynthesis
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Molecular Sequence Data
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Molecular Structure
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Multigene Family
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Open Reading Frames
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Sequence Homology, Amino Acid
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Streptomyces / enzymology
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Streptomyces / genetics*
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Tylosin / biosynthesis*
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Tylosin / chemistry
Substances
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Anti-Bacterial Agents
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DNA, Bacterial
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mycaminose
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Glucosamine
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Tylosin
Associated data
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GENBANK/D13307
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GENBANK/D13308
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GENBANK/L28170
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GENBANK/M74717
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GENBANK/U10405
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GENBANK/U37135
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GENBANK/X06150
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GENBANK/X63451
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GENBANK/X81885
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GENBANK/Z48268