A conserved infection pathway for filamentous bacteriophages is suggested by the structure of the membrane penetration domain of the minor coat protein g3p from phage fd

Structure. 1997 Feb 15;5(2):265-75. doi: 10.1016/s0969-2126(97)00184-6.

Abstract

Background: . Gene 3 protein (g3p), a minor coat protein from bacteriophage fd mediates infection of Escherichia coli bearing an F-pilus. Its N-terminal domain (g3p-D1) is essential for infection and mediates penetration of the phage into the host cytoplasm presumbly through interaction with the Tol complex in the E. coli membranes. Structural knowledge of g3p-D1 is both important for a molecular understanding of phage infection and of biotechnological relevance, as g3p-D1 represents the primary fusion partner in phage display technology.

Results: . The solution structure of g3p-D1 was determined by NMR spectroscopy. The principal structural element of g3p-D1 is formed by a six-stranded beta barrel topologically identical to a permutated SH3 domain but capped by an additional N-terminal alpha helix. The presence of structurally similar domains in the related E. coli phages, lke and 12-2, as well as in the cholera toxin transducing phage ctxφ is indicated. The structure of g3p-D1 resembles those of the recently described PTB and PDZ domains involved in eukaryotic signal transduction.

Conclusions: . The predicted presence of similar structures in membrane penetration domains from widely diverging filamentous phages suggests they share a conserved infection pathway. The widespread hydrogen-bond network within the beta barrel and N-terminal alpha helix in combination with two disulphide bridges renders g3p-D1 a highly stable domain, which may be important for keeping phage infective in harsh extracellular environments.

Publication types

  • Comparative Study

MeSH terms

  • Adsorption
  • Amino Acid Sequence
  • Base Sequence
  • Capsid Proteins
  • Cell Membrane / virology
  • Coliphages / chemistry
  • Coliphages / physiology*
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / physiology
  • Escherichia coli / virology
  • Fimbriae, Bacterial / virology
  • Magnetic Resonance Spectroscopy
  • Models, Molecular
  • Molecular Sequence Data
  • Protein Structure, Tertiary*
  • Sequence Homology, Amino Acid
  • Structure-Activity Relationship
  • Viral Fusion Proteins / chemistry*
  • Viral Fusion Proteins / physiology

Substances

  • Capsid Proteins
  • DNA-Binding Proteins
  • Viral Fusion Proteins