Mapping and Characterization of the Origin of DNA Replication of Porcine Circovirus

J Virol. 1997 Mar;71(3):2562-6.

Abstract

The origin of DNA replication of porcine circovirus (PCV) was mapped to a 111-bp fragment. On top of a hairpin, a nonanucleotide (TAGTATTAC) homologous to nonanucleotides of other viruses was identified. Mutation of this element abolishes replication. PCV may be related to a virus family characterized by single-stranded circular DNA genomes, rolling-circle replication, and homology of their rep proteins.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Chromosome Mapping*
  • Circovirus / genetics*
  • DNA, Viral*
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Replication Origin*
  • Sequence Homology, Amino Acid
  • Swine

Substances

  • DNA, Viral

Associated data

  • GENBANK/Y09921