Effect of congenital hypothyroidism on cell density in the ganglion cell layer of the rat retina

Braz J Med Biol Res. 1996 May;29(5):665-8.


The effect of congenital hypothyroidism on the visual system of Wistar rats was studied by determining neuron density in the retinal ganglion cell layer. Retinae of adult rats from mothers treated with propylthiouracil, 50 mg/day, starting on the 15th day of pregnancy (PTU group), and of adult rats from untreated mothers (control group) were examined. Retinae were prepared, and the neurons in the nasotemporal region located above the optic disc were counted. Hypothyroid rats showed a significant reduction in the retinal area (about 6.8%), when compared to controls. The cell density in the retinal ganglion cell layer was significantly decreased in 6 PTU-treated compared to 5 control retinae in total (2,793 +/- 330 vs 3,704 +/- 662 neurons/mm2), nasal (3,031 +/- 580 vs 3,853 +/- 699 neurons/mm2) and temporal (2,555 +/- 155 vs 3,555 +/- 827 neurons/mm2) regions. These alterations in a region considered to be one of the most specialized in the visual process suggest a structural deficiency induced by congenital hypothyroidism, with a possible decrease in the visual acuity of the rat.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Count
  • Congenital Hypothyroidism*
  • Female
  • Hypothyroidism / chemically induced
  • Hypothyroidism / complications
  • Male
  • Pregnancy
  • Propylthiouracil / pharmacology*
  • Rats
  • Rats, Wistar
  • Retina / cytology*
  • Retinal Ganglion Cells / cytology*
  • Thyroid Hormones / blood
  • Visual Acuity


  • Thyroid Hormones
  • Propylthiouracil