Classification of catechol 1,2-dioxygenase family: sequence analysis of a gene for the catechol 1,2-dioxygenase showing high specificity for methylcatechols from Gram+ aniline-assimilating Rhodococcus erythropolis AN-13

Gene. 1997 Jan 31;185(1):49-54. doi: 10.1016/s0378-1119(96)00629-4.


Gram+ aniline-assimilating Rhodococcus erythropolis AN-13 (AN-13) produces catechol 1,2-dioxygenase (C12O) showing high enzymatic activities for 3- and 4-methylcatechols [Aoki et al. (1984) Agric. Biol. Chem. 48, 2087-2095]. A 3.0 kb Sau3AI fragment carrying a gene encoding C12O(catA) was cloned by selection of transformants showing C12O activity from a gene library of AN-13. Furthermore, we specified a 1.6 kb SalI fragment containing catA from the Sau3AI fragment by subcloning. Sequence analysis revealed that the 1.6 kb SalI fragment carried a 855 bp open reading frame (ORF) encoding the entire AN-13 catA, preceded by a potential ribosome binding site (RBS). From comparison of the deduced amino acid (aa) sequence of C12O from AN-13 with other C12O reported previously, it was found that the AN-13 enzyme shares 56.0% aa sequence identity with C12o from Arthrobacter sp. mA3 (mA3) [Eck and Belter (1991) Gene 123, 87-92] compared with less than 36.4% aa sequence identities with others. In conclusion, we classified all C12O including the AN-13 enzyme into three subfamilies on the basis of similarity of aa sequences, numbers of aa residues, and substrate specificity.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Arthrobacter / genetics
  • Base Sequence
  • Binding Sites / genetics
  • Catechol 1,2-Dioxygenase
  • Catechols / metabolism
  • Cloning, Molecular
  • Dioxygenases*
  • Gene Expression Regulation, Bacterial
  • Gene Expression Regulation, Enzymologic
  • Gene Library
  • Molecular Sequence Data
  • Open Reading Frames
  • Oxygenases / classification*
  • Oxygenases / genetics*
  • Oxygenases / metabolism
  • Restriction Mapping
  • Rhodococcus / enzymology*
  • Rhodococcus / genetics*
  • Ribosomes / genetics
  • Sequence Homology, Amino Acid
  • Substrate Specificity / genetics
  • Transformation, Genetic


  • Catechols
  • 3-methylcatechol
  • 4-methylcatechol
  • Oxygenases
  • Dioxygenases
  • Catechol 1,2-Dioxygenase

Associated data

  • GENBANK/D83237