Molecular cloning of gilthead seabream (Sparus aurata) pituitary transcription factor GHF-1/Pit-1

Gene. 1997 Jan 31;185(1):87-93. doi: 10.1016/s0378-1119(96)00634-8.

Abstract

We report here the complete nucleotide sequence of a cDNA clone encoding Sparus aurata GHF-1/Pit-1 isolated from an expression library prepared from gilthead seabream pituitary gland poly(A)+ RNA. The cDNA sequence (saGHF-1/Pit-1) encodes a protein of 371 amino acids (aa) containing a POU domain (aa 194-343) and a transactivation, STA domain (aa 1-128). Northern blot hybridization of pituitary RNA detected a single 3.0 kb band and a rat GHF-1/Pit-1 antiserum was found to immunoreact with pituitary protein species of 42 kDa by Western blot analysis. When compared with mammalian GHF-1/Pit-1 aa sequence, the POU and STA domains of saGHF-1/Pit-1 protein show 83% and 48% aa identity, respectively. In spite of the low homology of the transactivation domain, saGHF-1/Pit-1 is able to activate the transcription of the human growth hormone promoter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Blotting, Western
  • Cloning, Molecular*
  • DNA, Complementary / genetics
  • Gene Expression Regulation
  • Gene Library
  • HeLa Cells
  • Human Growth Hormone / genetics
  • Humans
  • Molecular Sequence Data
  • Perciformes / genetics*
  • Pituitary Gland / chemistry*
  • Pituitary Gland / metabolism
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • RNA / analysis
  • Rats
  • Sequence Homology, Amino Acid
  • Trans-Activators / genetics
  • Transcription Factors / genetics*
  • Transcription Factors / immunology
  • Transcription, Genetic

Substances

  • DNA, Complementary
  • Trans-Activators
  • Transcription Factors
  • Human Growth Hormone
  • RNA

Associated data

  • GENBANK/X81646