Molecular cloning of a gene required for DNA replication in Ustilago maydis

Mol Gen Genet. 1997 Jan 27;253(4):463-8. doi: 10.1007/s004380050344.


TSD2, a gene necessary for DNA synthesis in Ustilago maydis, was cloned by complementation of the temperature sensitive growth defect of a mutant known previously as pol1-1 and renamed here tsd2-1. Linkage analysis established that the cloned fragment contained an allele of tsd2-1 and not a suppressor. DNA sequence determination of the cloned DNA fragment indicated the presence of a single large uninterrupted open reading frame capable of encoding a protein of 845 amino acids without homology to any known gene involved in DNA synthesis. TSD2 was found to be cell cycle-regulated and mRNA levels peaked in early S or G1 phase.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Cycle Proteins / chemistry
  • Cell Cycle Proteins / genetics*
  • Cloning, Molecular
  • DNA Replication / genetics*
  • DNA, Fungal / biosynthesis
  • DNA, Fungal / genetics
  • Fungal Proteins*
  • G1 Phase
  • Gene Expression Regulation, Fungal
  • Genes, Fungal*
  • Genetic Complementation Test
  • Molecular Sequence Data
  • Open Reading Frames
  • RNA, Fungal / genetics
  • RNA, Fungal / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • S Phase
  • Sequence Analysis, DNA
  • Ustilago / cytology
  • Ustilago / genetics*
  • Ustilago / metabolism


  • Cell Cycle Proteins
  • DNA, Fungal
  • Fungal Proteins
  • RNA, Fungal
  • RNA, Messenger
  • TSD2 protein, Ustilago maydis

Associated data

  • GENBANK/U50276