Abstract
A cDNA coding for a newly identified Bermuda grass pollen allergen, Cyn d 7, with significant sequence similarity to Ca2+-binding proteins, was isolated from a cDNA expression library using serum IgE from an allergic individual. The deduced amino acid sequence of Cyn d 7 contained two typical Ca2+-binding sites (EF hand domains). Depletion of Ca2+ with EGTA led to a loss of IgE-binding capacity of rCyn d 7. A synthetic peptide based on domain II showed high IgE reactivity. Cyn d 7 therefore represents a grass pollen allergen that belongs to a novel class of Ca2+-binding proteins.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Allergens*
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Amino Acid Sequence
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Antigens, Plant
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Binding Sites
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Calcium / metabolism
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Calcium-Binding Proteins / biosynthesis
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Calcium-Binding Proteins / chemistry
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Calcium-Binding Proteins / immunology*
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Cloning, Molecular
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Humans
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Immunoglobulin E / blood
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Molecular Sequence Data
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Peptides / chemical synthesis
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Peptides / chemistry
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Peptides / immunology
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Plant Proteins*
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Poaceae / immunology*
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Poaceae / metabolism
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Pollen
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Recombinant Proteins / immunology
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Sequence Homology, Amino Acid
Substances
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Allergens
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Antigens, Plant
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Calcium-Binding Proteins
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Cyn d 7 protein, Cupressus arizonica
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Peptides
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Plant Proteins
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Recombinant Proteins
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Immunoglobulin E
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Calcium
Associated data
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GENBANK/U35683
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GENBANK/X91256