Neurotrophin-3 promotes the survival of oligodendrocyte precursors in embryonic hippocampal cultures under chemically defined conditions

Brain Res. 1997 Jan 23;746(1-2):19-24. doi: 10.1016/s0006-8993(96)01199-7.

Abstract

embryonic rat hippocampal cells were cultured in basal medium with or without addition of the neurotrophin NT-3. After culturing in these extreme conditions, the effects of NT-3 on the neuronal and on the glial components were assessed. Neurons survived even in the absence of NT-3 but failed to reach terminal differentiation. On the other hand, NT-3 promoted the survival but not the proliferation and/or the differentiation of oligodendrocytes precursors present in the same culture, an effect that was reversed by the addition of neutralizing antibodies against NT-3. Type I or II astrocytes were not affected by NT-3. These results reinforce the role for NT-3 in oligodendrocyte lineage development and allow to dissect the roles of this neurotrophin in survival and in proliferation/differentiation of oligodendrocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation / drug effects
  • Cell Survival / drug effects
  • Cells, Cultured / drug effects
  • Culture Media / pharmacology
  • Fluorescent Antibody Technique
  • Hippocampus / cytology
  • Hippocampus / embryology
  • Nerve Growth Factors / pharmacology*
  • Neurons / cytology
  • Neurons / drug effects
  • Neurotrophin 3
  • Oligodendroglia / cytology
  • Oligodendroglia / drug effects*
  • Rats
  • Stem Cells / cytology
  • Stem Cells / drug effects*

Substances

  • Culture Media
  • Nerve Growth Factors
  • Neurotrophin 3