Presence of human herpesvirus 8 variants in the oral tissues of human immunodeficiency virus-infected persons

J Infect Dis. 1997 Mar;175(3):703-7. doi: 10.1093/infdis/175.3.703.

Abstract

A 210-bp DNA segment specific to the human herpesvirus 8 (HHV-8) genome was amplified by nested polymerase chain reaction from 10 of 14 archived oral biopsy samples of HIV-positive patients in London who had no evidence of oral Kaposi's sarcoma (KS). Various oral sites were represented. Oral tissues from 20 general dental patients not known to be HIV-infected were negative. When DNA sequences of these products were compared with sequences derived from 5 oral KS tissues of AIDS patients in London and 10 skin biopsies of Italian patients with Mediterranean KS (total number of positive tissues = 25), 11 were found to be unique. DNA and predicted peptide motifs of these sequences were also different from those in 28 of 36 HHV-8-positive lesions previously reported from American and African patients. HHV-8 is tropic for the oral mucosa of HIV-infected persons, and HHV-8 variants, though diverse, may be geographically restricted.

MeSH terms

  • Amino Acid Sequence
  • Biopsy
  • Carrier State
  • DNA, Viral / analysis*
  • HIV Infections / microbiology*
  • Herpesvirus 8, Human / classification
  • Herpesvirus 8, Human / genetics*
  • Humans
  • Molecular Sequence Data
  • Mouth Mucosa / microbiology*
  • Sarcoma, Kaposi / microbiology
  • Viral Proteins / classification
  • Viral Proteins / genetics

Substances

  • DNA, Viral
  • Viral Proteins