Abstract
A plasma PCR test, using a nonradioactive PCR plate assay, was evaluated for detection of human cytomegalovirus reactivation. This assay was compared to Southern blotting and found to perform well. As a noncompetitive method of quantitation, it was similar to a competitive method for detecting the number of genome copies per milliliter of plasma in marrow transplant recipients. This is a technically simplified assay with potential for adaptation to automation.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Bone Marrow Transplantation / adverse effects*
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Cytomegalovirus / genetics*
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Cytomegalovirus / isolation & purification*
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Cytomegalovirus Infections / diagnosis*
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Cytomegalovirus Infections / etiology
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Cytomegalovirus Infections / virology*
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DNA Probes / genetics
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DNA, Viral / blood
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DNA, Viral / genetics
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Humans
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Polymerase Chain Reaction / methods*
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Virology / methods