Chicken ovalbumin upstream promoter--transcription factor (COUP-TF) expression in human endometrial cancer cell lines

Anticancer Res. Nov-Dec 1996;16(6B):3371-6.

Abstract

COUP-TF is an orphan member of the steroid receptor superfamily. COUP-TF down-regulates hormonal induction by other steroid receptors involved in cell proliferation and differentiation. Previous study has suggested a role in gynecological adenocarcinoma. In the present study we evaluated COUP-TF expression in endometrial cancer. Fourteen permanent endometrial cancer cell lines were established front the primary site of 14 endometrial cancer patients. Immunocytochemistry for COUP-TF-like activity was performed using an affinity selected polyclonal rabbit-derived antibody in an immunoperoxidase staining technique. The staining intensity and cell surface area were quantified by image analysis. By immunostain 2 cell lines were COUP-TF (+), 6 (+ +) and 6 (+ + +). Quantitative differences in staining intensity and cell surface area were not significant in these groups. All cell lines tested were immunocytochemically negative for estrogen and progesterone receptors. COUP-TF is a new factor involved in endometrial cancer cell differentiation and growth, especially in estrogen receptor negative tumors.

MeSH terms

  • Animals
  • COUP Transcription Factor I
  • DNA-Binding Proteins / metabolism*
  • Endometrial Neoplasms / metabolism*
  • Female
  • Humans
  • Immunohistochemistry
  • Neoplasm Proteins / metabolism*
  • Transcription Factors / metabolism*
  • Tumor Cells, Cultured / metabolism

Substances

  • COUP Transcription Factor I
  • DNA-Binding Proteins
  • NR2F1 protein, human
  • Neoplasm Proteins
  • Transcription Factors