Effect of capsianoside, a diterpene glycoside, on tight-junctional permeability

Biochim Biophys Acta. 1997 Jan 31;1323(2):281-90. doi: 10.1016/s0005-2736(96)00196-4.


Previous work (Hashimoto et al., (1994) Biosci. Biotech. Biochem. 58, 1345) revealed that a sweet pepper extract enhanced the tight-junctional (TJ) permeability of a human intestinal Caco-2 cell monolayer. In the present study, the substance which modulated the TJ permeability was chromatographically purified from the extract. The active substances were identified as capsianosides A-F, diterpene glycosides. Treatment of the cells with capsianoside F, the most active compound, decreased the cellular G-actin content by 40% and increased the F-actin content by 16%. The effect of capsianoside F was significantly suppressed by disturbing the cytoskeletal structure with cytochalasin D at a low dose (50 ng/ml). These results suggest that capsianosides affected the cytoskeletal function by modulating the reorganization of actin filaments, by which the TJ structure and permeability were changed. The possible involvement of a PKC inhibition in the mechanism of an increase in TJ permeability is also suggested.

MeSH terms

  • Actins / analysis
  • Caco-2 Cells
  • Cell Membrane Permeability / drug effects*
  • Chelating Agents
  • Cytoskeleton / drug effects
  • Diterpenes / chemistry
  • Diterpenes / isolation & purification
  • Diterpenes / pharmacology*
  • Glycosides / chemistry
  • Glycosides / pharmacology*
  • Humans
  • Plant Extracts / chemistry
  • Poloxalene / pharmacology
  • Protein Kinase C / metabolism
  • Surface-Active Agents / pharmacology
  • Tight Junctions / drug effects*
  • Vegetables / chemistry


  • Actins
  • Chelating Agents
  • Diterpenes
  • Glycosides
  • Plant Extracts
  • Surface-Active Agents
  • Poloxalene
  • Protein Kinase C