The topoisomerase I gene from Candida albicans

Microbiology. 1997 Feb;143 ( Pt 2):377-86. doi: 10.1099/00221287-143-2-377.

Abstract

We report here the cloning of the Candida albicans genomic topoisomerase I gene (TOP1) by use of PCR and subsequent hybridization. The predicted protein sequence shared 58.8% identity with the Saccharomyces cerevisiae topoisomerase I and 30-50% identity with other eukaryotic topoisomerase I proteins. A conditional gene disruption strain (CWJ477) was constructed so that one copy of TOP1 was deleted and the other copy of TOP1 was placed under a regulatable promoter. Under repressed conditions, cells grew slowly and cell morphology was abnormal. The virulence of CWJ477 was markedly reduced in a mouse model system, and that of the single gene knockout-strain was slightly attenuated, indicating that TOP1 might play a role in the infection of C. albicans in mice in a dose-dependent manner. Despite the reduced virulence of both the single and double knockout strains, viable cells of the pathogen were recovered from the kidneys as late as 22 d post-infection.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Candida albicans / enzymology
  • Candida albicans / genetics*
  • Candida albicans / pathogenicity*
  • Cloning, Molecular
  • DNA Topoisomerases, Type I / genetics*
  • Gene Deletion
  • Genes, Fungal*
  • Genome, Fungal
  • Kidney / microbiology
  • Mice
  • Molecular Sequence Data
  • Morphogenesis
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Survival Analysis
  • Virulence / genetics

Substances

  • DNA Topoisomerases, Type I

Associated data

  • GENBANK/U41342