Identification of amdX, a New Cys-2-His-2 (C2H2) Zinc-Finger Gene Involved in the Regulation of the amdS Gene of Aspergillus Nidulans

Mol Microbiol. 1997 Feb;23(3):591-602. doi: 10.1046/j.1365-2958.1997.d01-1872.x.

Abstract

The acetamidase-encoding amdS gene of Aspergillus nidulans has been shown to be controlled by multiple regulatory genes. A new gene, amdX, involved in amdS regulation was identified during the characterization of a translocation affecting amdS control. The amdX gene is predicted to encode a 1150-amino-acid polypeptide which contains two Cys-2-His-2 (C2H2) zinc finger DNA-binding motifs. Insertional inactivation of amdX and the phenotypes of transformants containing multiple copies of the amdX gene show that it is an activator of amdS expression. A fusion protein containing the AmdX zinc fingers was found to bind to sequences in the 5' region of amdS which overlap binding sites for the CreA and AmdA regulatory proteins. Evidence is presented for AmdX acting at these sites in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Aspergillus nidulans / genetics*
  • Aspergillus nidulans / physiology
  • Base Sequence
  • DNA Footprinting
  • DNA Mutational Analysis
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / isolation & purification
  • DNA-Binding Proteins / physiology*
  • Fungal Proteins*
  • Gene Expression Regulation
  • Gene Expression Regulation, Fungal / physiology*
  • Genes, Fungal / genetics*
  • Genes, Fungal / physiology
  • Molecular Sequence Data
  • Phenotype
  • Restriction Mapping
  • Sequence Analysis
  • Sequence Homology, Amino Acid
  • Zinc Fingers / genetics*

Substances

  • AmdX protein, Emericella nidulans
  • DNA-Binding Proteins
  • Fungal Proteins

Associated data

  • GENBANK/U56100