CRADD, a novel human apoptotic adaptor molecule for caspase-2, and FasL/tumor necrosis factor receptor-interacting protein RIP

Cancer Res. 1997 Feb 15;57(4):615-9.

Abstract

FADD/MORT1 is a death domain (DD)-containing adaptor/signaling molecule that interacts with the intracellular DD of FAS/APO-I (CD95) and tumor necrosis factor receptor 1 and the prodomain of caspase-8 (Mch5/MACH/FLICE). FADD engagement of caspase-8 presumably activates this caspase and leads to apoptosis. Another DD-containing adaptor/signaling molecule, CRADD, was identified and was shown to induce apoptosis. CRADD has a dual-domain structure similar to that of FADD. It has an NH2-terminal caspase homology domain that interacts with caspase-2 and a COOH-terminal DD that interacts with RIP. CRADD is constitutively expressed in many tissues and thus could play a role in regulating apoptosis in mammalian cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing*
  • Adult
  • Amino Acid Sequence
  • Apoptosis / physiology*
  • Base Sequence
  • CRADD Signaling Adaptor Protein
  • Carrier Proteins / genetics*
  • Carrier Proteins / isolation & purification
  • Carrier Proteins / metabolism
  • Cysteine Endopeptidases / genetics
  • Cysteine Endopeptidases / metabolism
  • Fas Ligand Protein
  • Fas-Associated Death Domain Protein
  • Fetus
  • Humans
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism
  • Molecular Sequence Data
  • Proteins / genetics*
  • Proteins / isolation & purification
  • Proteins / metabolism
  • Receptor-Interacting Protein Serine-Threonine Kinases
  • Receptors, Tumor Necrosis Factor / genetics
  • Receptors, Tumor Necrosis Factor / metabolism
  • Sequence Homology, Amino Acid
  • fas Receptor / genetics

Substances

  • Adaptor Proteins, Signal Transducing
  • CRADD Signaling Adaptor Protein
  • CRADD protein, human
  • Carrier Proteins
  • FADD protein, human
  • FASLG protein, human
  • Fas Ligand Protein
  • Fas-Associated Death Domain Protein
  • Membrane Glycoproteins
  • Proteins
  • Receptors, Tumor Necrosis Factor
  • fas Receptor
  • RIPK1 protein, human
  • Receptor-Interacting Protein Serine-Threonine Kinases
  • Cysteine Endopeptidases

Associated data

  • GENBANK/U84388