Development of aberrant crypt foci involves a fission mechanism as revealed by isolation of aberrant crypts

Jpn J Cancer Res. 1996 Dec;87(12):1199-203. doi: 10.1111/j.1349-7006.1996.tb03133.x.


Morphological analysis of isolated colonic crypts in rats, postnatally, indicated that the crypts reproduce themselves by a fission mechanism, the division beginning at the crypt base and proceeding upwards until there are two separate crypts. Occasionally, before the separation is complete, a second fission process starts on one or both sides of a bifurcating crypt and a triple-branched or quadruple-branched crypt results. Analysis of isolated aberrant crypt foci (ACF) in rats treated with 1,2-dimethylhydrazine revealed that the development of ACF consisting of multiple crypts is also due to a fission mechanism. Initially, an indentation appears at the base of a single ACF crypt, with subsequent formation of a bifurcation and eventual crypt division.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1,2-Dimethylhydrazine
  • Animals
  • Colon / drug effects
  • Colon / pathology*
  • Colon / ultrastructure
  • Dimethylhydrazines / pharmacology
  • Microscopy, Electron, Scanning
  • Mutagens / pharmacology
  • Rats
  • Rats, Inbred F344


  • Dimethylhydrazines
  • Mutagens
  • 1,2-Dimethylhydrazine