Both E12 and E47 allow commitment to the B cell lineage

Immunity. 1997 Feb;6(2):145-54. doi: 10.1016/s1074-7613(00)80421-5.


The E2A gene products, E12 and E47, are required for proper B cell development. Mice lacking the E2A gene products generate only a very small number of B220+ cells, which lack immunoglobulin DJ(H) rearrangements. We have now generated mice expressing either E12 or E47. B cell development in mice expressing E12 but lacking E47 is perturbed at the pro-B cell stage, and these mice lack IgM+B220+ B cells in both bone marrow and spleen. IgM+B220+ B cells can be detected, albeit at significantly reduced levels, in the bone marrow and spleen of mice lacking E12. Ectopic expression of both E12 and E47 in a null mutant background shows that E12 and E47 act in concert to promote B lineage development. Taken together, the data indicate that both E12 and E47 allow commitment to the B cell lineage and act synergistically to promote B lymphocyte maturation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • B-Lymphocytes / immunology*
  • B-Lymphocytes / metabolism
  • Cell Differentiation / drug effects
  • Cell Differentiation / immunology
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / pharmacology
  • Drug Synergism
  • Gene Rearrangement, B-Lymphocyte / genetics
  • Gene Rearrangement, B-Lymphocyte / immunology
  • Mice
  • Mice, Mutant Strains
  • Mice, Transgenic
  • TCF Transcription Factors
  • Transcription Factor 7-Like 1 Protein
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics
  • Transcription Factors / pharmacology
  • Transcription, Genetic / genetics
  • Transcription, Genetic / immunology


  • DNA-Binding Proteins
  • TCF Transcription Factors
  • Tcf7l1 protein, mouse
  • Transcription Factor 7-Like 1 Protein
  • Transcription Factors