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Comparative Study
, 14 (2), 111-9

Comparative Flow Cytometric Analysis of the Human Sperm Acrosome Reaction Using CD46 Antibody and Lectins

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Comparative Study

Comparative Flow Cytometric Analysis of the Human Sperm Acrosome Reaction Using CD46 Antibody and Lectins

J A Carver-Ward et al. J Assist Reprod Genet.

Abstract

Purpose: Our purpose was to determine the most suitable marker for the human sperm acrosome reaction, based on detection of CD46 antibody binding compared with lectin binding.

Methods: Flow cytometric analysis of CD46 antibody versus lectins (PNA, PSA, and Con A) was used to quantify the acrosome reaction of human sperm.

Results: Neither PSA nor Con A was able to detect significant changes in the spontaneous and ionophore-induced acrosome reactions compared to CD46 antibody. However, PNA was found to exhibit a binding pattern similar to that observed with CD46 and could be used to quantify measurable changes in acrosomal response to ionophore, albeit of a lower magnitude than the responses detected by CD46.

Conclusions: We conclude that PNA binds to the inner acrosomal membrane of acrosome-reacted sperm and is suitable for use as a marker of the acrosome reaction by flow cytometry. Data are presented which clarify the assessment of the acrosome reaction when CD46 and lectins are used.

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