A human cDNA encoding the homologue of NADH: ubiquinone oxidoreductase subunit B13

Biochim Biophys Acta. 1997 Feb 7;1350(2):115-8. doi: 10.1016/s0167-4781(96)00208-4.

Abstract

A cDNA encoding the human homologue of bovine NADH:ubiquinone oxidoreductase (complex I of mitochondrial respiratory chain) subunit B13 has been isolated. The clone contains an open reading frame of 348 bp, 23 bp of 5'-untranslated sequence (UTR) and a long 3'UTR of 1088 bp. The deduced amino-acid sequence is 87% identical to bovine B13. Human B13 mRNA expression was observed in all tissues examined with highest levels in heart, skeletal muscle and brain. Southern analysis of human genomic DNA revealed the presence of multigene family.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / metabolism
  • Cattle
  • Cloning, Molecular
  • DNA Primers / genetics
  • DNA, Complementary / genetics*
  • Electron Transport Complex I
  • Gene Expression
  • Humans
  • Molecular Sequence Data
  • Muscle, Skeletal / metabolism
  • Myocardium / metabolism
  • NADH, NADPH Oxidoreductases / chemistry
  • NADH, NADPH Oxidoreductases / genetics*
  • Open Reading Frames
  • Protein Conformation
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Species Specificity
  • Tissue Distribution

Substances

  • DNA Primers
  • DNA, Complementary
  • RNA, Messenger
  • NADH, NADPH Oxidoreductases
  • Electron Transport Complex I

Associated data

  • GENBANK/U53468
  • GENBANK/Z68493
  • SWISSPROT/P24919
  • SWISSPROT/P80266