Cloning and expression of human cDNA encoding Na+, K(+)-ATPase gamma-subunit

Biochim Biophys Acta. 1997 Feb 7;1350(2):133-5. doi: 10.1016/s0167-4781(96)00219-9.

Abstract

A cDNA clone encoding the Na+, K(+)-Atpase gamma-subunit polypeptide was cloned from a human fetal liver cDNA library. The deduced amino acid sequence of the protein comprised 58 amino acids with a calculated molecular weight of 6400 Da, and showed about 86% homology when compared with those of the bovine, rat and mouse Na+, K(+)-ATPase gamma-subunits published elsewhere. Northern blot analysis showed that the cDNA hybridized to a 0.7 kb mRNA which was expressed in human kidney, pancreas and fetal liver.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cattle
  • Cloning, Molecular
  • DNA, Complementary / genetics*
  • Fetus / metabolism
  • Humans
  • Kidney / metabolism
  • Liver / metabolism
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Pancreas / metabolism
  • Protein Conformation
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Sequence Homology, Amino Acid
  • Sodium-Potassium-Exchanging ATPase / chemistry
  • Sodium-Potassium-Exchanging ATPase / genetics*
  • Tissue Distribution

Substances

  • DNA, Complementary
  • RNA, Messenger
  • Sodium-Potassium-Exchanging ATPase