Laryngeal and oropharyngeal cancer, and alcohol dehydrogenase 3 and glutathione S-transferase M1 polymorphisms

Hum Genet. 1997 Mar;99(3):319-25. doi: 10.1007/s004390050365.


In this study the GSTmu phenotype and ADH genotype at the ADH3 locus were investigated in a group of 39 alcoholic men with upper respiratory/digestive tract cancer: 21 with oropharyngeal cancer and 18 with laryngeal cancer. The results are compared with those of a control group of 37 alcoholic men without alcohol-related medical complications. Of the control subjects, 48% were found to be GSTmu deficient [GSTmu(-)] and 19% carried the ADH(3)1/ADH(3)1 genotype. In the laryngeal cancer patients, a significantly elevated frequency of both the GSTmu(-) (78%) and ADH(3)1/ADH(3)1 genotype (56%) was observed, relative to the control group. On the basis of this result, the risk of laryngeal cancer associated with the GSTmu(-) and ADH(3)1/ADH(3)1 genotypic combination within the population of alcoholics was estimated to be 12.9 with a 95% confidence interval of 1.8-92 (P < 0.01) relative to alcoholic individuals who have GSTmu [GSTmu(+)] and are not ADH(3)1/ADH(3)1. Thus, alcoholics who are GSTmu(-) and ADH(3)1/ADH(3)1 have at least an 80% greater risk of developing laryngeal cancer than alcoholics who are GSTmu(+) and who are not ADH(3)1/ADH(3)1. In addition, the oropharyngeal cancer patients had excess frequencies of both GSTmu(-) (62%) and ADH(3)1/ADH(3)1 (43%) relative to the control group, but these excess frequencies were not statistically significant. The GSTmu(-) and ADH(3)1/ADH(3)1 genotypic combination may be a constitutional risk factor for laryngeal cancer among alcoholics.

MeSH terms

  • Adult
  • Alcohol Dehydrogenase / genetics*
  • Alcoholism / complications
  • Case-Control Studies
  • Genotype
  • Glutathione Transferase / genetics*
  • Humans
  • Laryngeal Neoplasms / enzymology
  • Laryngeal Neoplasms / etiology
  • Laryngeal Neoplasms / genetics*
  • Male
  • Middle Aged
  • Oropharyngeal Neoplasms / enzymology
  • Oropharyngeal Neoplasms / etiology
  • Oropharyngeal Neoplasms / genetics*
  • Risk Factors


  • Alcohol Dehydrogenase
  • Glutathione Transferase