Induction of human cytotoxic T lymphocytes specific for prostate-specific antigen

Prostate. 1997 Feb 1;30(2):73-8. doi: 10.1002/(sici)1097-0045(19970201)30:2<73::aid-pros1>;2-l.


Prostate-specific antigen (PSA), a tissue-specific protein expressed by most adenocarcinomas of the prostate, might be a useful target for T-cell-mediated immunotherapy of prostate cancers. The current study examined whether it is possible to elicit human cytotoxic T lymphocytes (CTL) with specificity for PSA. A synthetic nonamer peptide, corresponding to residues 146-154 of PSA and containing a canonical HLA-A2-binding motif, was shown to stabilize the expression of HLA-A2 on the T2 antigen-processing mutant cell line. Repeated in vitro stimulation of peripheral blood lymphocytes from a normal HLA-A2+ donor induced CTL with specificity for the PSA 146-154 peptide. The peptide-induced CTL expressed the CD4- CD8+ cell surface phenotype and were restricted by HLA-A2. A large portion of patients with prostate cancer express the HLA-A2 phenotype, implying that many prostate cancers might be targeted by HLA-A2-restricted CTL with specificity for the PSA 146-154 epitope.

MeSH terms

  • Adenocarcinoma / immunology*
  • Adenocarcinoma / therapy
  • Epitopes / chemistry
  • Epitopes / immunology
  • HLA-A2 Antigen / immunology*
  • Humans
  • Immunity, Cellular
  • Immunization
  • Immunotherapy
  • Male
  • Peptide Fragments / chemistry
  • Peptide Fragments / immunology*
  • Peptides / chemistry
  • Peptides / immunology
  • Prostate-Specific Antigen / chemistry
  • Prostate-Specific Antigen / immunology*
  • Prostatic Neoplasms / immunology*
  • Prostatic Neoplasms / therapy
  • T-Lymphocytes, Cytotoxic / immunology*
  • Tumor Cells, Cultured


  • Epitopes
  • HLA-A2 Antigen
  • Peptide Fragments
  • Peptides
  • prostate-specific antigen (146-154)
  • Prostate-Specific Antigen