Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites

Protein Eng. 1997 Jan;10(1):1-6. doi: 10.1093/protein/10.1.1.


We have developed a new method for the identification of signal peptides and their cleavage sites based on neural networks trained on separate sets of prokaryotic and eukaryotic sequence. The method performs significantly better than previous prediction schemes and can easily be applied on genome-wide data sets. Discrimination between cleaved signal peptides and uncleaved N-terminal signal-anchor sequences is also possible, though with lower precision. Predictions can be made on a publicly available WWW server.

MeSH terms

  • Algorithms
  • Amino Acid Sequence / physiology*
  • ErbB Receptors / chemistry
  • Eukaryotic Cells / chemistry*
  • Humans
  • Molecular Sequence Data
  • Neural Networks, Computer*
  • Prokaryotic Cells / chemistry*
  • Protein Processing, Post-Translational / physiology
  • Protein Sorting Signals / chemistry*
  • Structure-Activity Relationship


  • Protein Sorting Signals
  • ErbB Receptors