Abstract
In Xenopus laevis embryos, the Wingless/Wnt-1 subclass of Wnt molecules induces axis duplication, whereas the Wnt-5A subclass does not. This difference could be explained by distinct signal transduction pathways or by a lack of one or more Wnt-5A receptors during axis formation. Wnt-5A induced axis duplication and an ectopic Spemann organizer in the presence of hFz5, a member of the Frizzled family of seven-transmembrane receptors. Wnt-5A/hFz5 signaling was antagonized by glycogen synthase kinase-3 and by the amino-terminal ectodomain of hFz5. These results identify hFz5 as a receptor for Wnt-5A.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
-
Animals
-
Calcium-Calmodulin-Dependent Protein Kinases / antagonists & inhibitors
-
Calcium-Calmodulin-Dependent Protein Kinases / metabolism
-
DNA-Binding Proteins / genetics
-
Drosophila Proteins*
-
Embryonic Development*
-
Embryonic Induction*
-
Frizzled Receptors
-
Glycogen Synthase Kinase 3
-
Glycogen Synthase Kinases
-
Goosecoid Protein
-
Homeodomain Proteins*
-
Humans
-
Ligands
-
Membrane Proteins / chemistry
-
Membrane Proteins / genetics
-
Membrane Proteins / metabolism*
-
Proteins / genetics
-
Proteins / metabolism*
-
Receptors, Cell Surface / genetics
-
Receptors, Cell Surface / metabolism*
-
Receptors, G-Protein-Coupled
-
Repressor Proteins*
-
Signal Transduction
-
Transcription Factors*
-
Wnt Proteins
-
Wnt-5a Protein
-
Xenopus Proteins*
-
Xenopus laevis / embryology
Substances
-
DNA-Binding Proteins
-
Drosophila Proteins
-
Frizzled Receptors
-
Goosecoid Protein
-
Homeodomain Proteins
-
Ligands
-
Membrane Proteins
-
Proteins
-
Receptors, Cell Surface
-
Receptors, G-Protein-Coupled
-
Repressor Proteins
-
Transcription Factors
-
Wnt Proteins
-
Wnt-5a Protein
-
Wnt5a protein, Xenopus
-
Xenopus Proteins
-
fz protein, Drosophila
-
Glycogen Synthase Kinases
-
Calcium-Calmodulin-Dependent Protein Kinases
-
Glycogen Synthase Kinase 3