Phosphorylation of the human La antigen on serine 366 can regulate recycling of RNA polymerase III transcription complexes

Cell. 1997 Mar 7;88(5):707-15. doi: 10.1016/s0092-8674(00)81913-3.

Abstract

The human La antigen is an RNA-binding protein that facilitates transcriptional termination and reinitiation by RNA polymerase III. Native La protein fractionates into transcriptionally active and inactive forms that are unphosphorylated and phosphorylated at serine 366, respectively, as determined by enzymatic and mass spectrometric analyses. Serine 366 comprises a casein kinase II phosphorylation site that resides within a conserved region in the La proteins from several species. RNA synthesis from isolated transcription complexes is inhibited by casein kinase II-mediated phosphorylation of La serine 366 and is reversible by dephosphorylation. This work demonstrates a novel mechanism of transcriptional control at the level of recycling of stable transcription complexes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoantigens / genetics*
  • Autoantigens / isolation & purification
  • Autoantigens / metabolism*
  • Blotting, Western
  • Casein Kinase II
  • Cell Fractionation
  • DNA-Binding Proteins / isolation & purification
  • DNA-Binding Proteins / metabolism
  • HeLa Cells / enzymology
  • Humans
  • Isoelectric Focusing
  • Mass Spectrometry
  • Molecular Sequence Data
  • Peptide Mapping
  • Phosphorylation
  • Protein-Serine-Threonine Kinases / isolation & purification
  • Protein-Serine-Threonine Kinases / metabolism
  • RNA Polymerase III / genetics*
  • RNA Polymerase III / metabolism*
  • Ribonucleoproteins / genetics*
  • Ribonucleoproteins / isolation & purification
  • Ribonucleoproteins / metabolism*
  • Sequence Homology, Amino Acid
  • Serine / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / isolation & purification
  • Transcription Factors / metabolism
  • Transcription, Genetic / physiology

Substances

  • Autoantigens
  • DNA-Binding Proteins
  • Ribonucleoproteins
  • SS-B antigen
  • Transcription Factors
  • Serine
  • Casein Kinase II
  • Protein-Serine-Threonine Kinases
  • RNA Polymerase III