Abstract
The cDNA for a novel gene, PL48, isolated by subtractive hybridization between undifferentiated human term cytotrophoblast and differentiating cytotrophoblast, has been cloned and sequenced. PL48 contains an open reading frame coding for a 537-amino acid protein, has multiple potential PKC, casein kinase II, and cAMP/cGMP-dependent kinase phosphorylation sites, and N-linked glycosylation sites. It is not present in a wide variety of proliferating cancer cells, but PL48 mRNA shows marked expression during cytotrophoblast and granulocyte lineage-specific HL-60 promyelocytic cell differentiation induced by DMSO.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding Sites
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Cell Adhesion Molecules
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Cell Differentiation / genetics
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Cell Lineage / genetics
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Cloning, Molecular
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Dimethyl Sulfoxide / pharmacology
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Female
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Gene Expression Regulation, Developmental
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Gene Expression Regulation, Neoplastic
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Genes, Tumor Suppressor
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HL-60 Cells / cytology*
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HL-60 Cells / drug effects
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HL-60 Cells / metabolism
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Humans
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Molecular Sequence Data
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Phosphorylation
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Placenta / metabolism
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Pregnancy
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Protein Conformation
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Protein Kinase C / metabolism
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Proteins / chemistry
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Proteins / genetics*
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Proteins / metabolism
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RNA, Messenger / biosynthesis
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RNA, Messenger / drug effects
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Transcription, Genetic
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Trophoblasts / cytology*
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Trophoblasts / metabolism
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Tumor Cells, Cultured
Substances
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Cell Adhesion Molecules
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Proteins
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RIPOR2 protein, human
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RNA, Messenger
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Protein Kinase C
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Dimethyl Sulfoxide