Virulence properties of Escherichia coli strains belonging to enteropathogenic (EPEC) serogroups isolated from calves with diarrhea

Vet Microbiol. 1997 Feb;54(2):145-53. doi: 10.1016/s0378-1135(96)01278-3.

Abstract

Nineteen Escherichia coli strains belonging to enteropathogenic (EPEC) serogroups were isolated from calves with diarrhea in Paraná State. Brazil, and studied for virulence markers associated with EPEC or enterohemorrhagic E. coli (EHEC). The 19 isolates belonged to 12 serotypes with isolates of O26:H11, O119:H25 and O114:H- being the most prevalent Localized adherence (LA) was demonstrated for 37% of the isolates, consisting of all four O26:H11, both O114:H- and one O114:H40 isolates. All the LA strains were positive in the fluorescent-actin staining (FAS) test and possessed attaching-effacing E. coli (eae) sequences, but only O114 strains hybridized with the EPEC adherence factor (EAF) probe. None of the strains produced Shiga-like toxins (Verotoxin). Only the O26:H11 strains hybridized with the EHEC plasmid specific (CVD419) probe and were enterohemolytic, properties associated with EHEC strains. This investigation demonstrates that among the bovine strains isolated only those of serogroup O114 behaved as typical EPEC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Bacterial Adhesion
  • Cattle
  • Cattle Diseases*
  • Diarrhea / microbiology
  • Diarrhea / veterinary*
  • Escherichia coli / classification*
  • Escherichia coli / isolation & purification
  • Escherichia coli / pathogenicity*
  • Escherichia coli Infections / microbiology
  • Escherichia coli Infections / physiopathology
  • Escherichia coli Infections / veterinary*
  • Feces / microbiology
  • HeLa Cells
  • Humans
  • Microbial Sensitivity Tests
  • Plasmids*
  • Reference Values
  • Serotyping
  • Virulence

Substances

  • Anti-Bacterial Agents