Effect of bacteria on survival and growth of Acanthamoeba castellanii

Curr Microbiol. 1997 Apr;34(4):212-5. doi: 10.1007/s002849900170.


The growth and survival of Acanthamoeba castellanii in the presence of Gram-negative bacteria such as Pseudomonas aeruginosa, Escherichia coli, Serratia marcescens, and Stenotrophomonas maltophilia varied with the densities and species of bacteria. All species of bacteria suspended in a buffered saline at densities of 10(5) to 10(6)/ml supported the growth and survival of 10(6)/ml trophozoites of Acanthamoeba castellanii in a buffered saline solution. At densities of bacteria to amoebae of 100:1 or greater, growth and survival of A. castellanii were suppressed, particularly by P. aeruginosa. In an enrichment medium, the rapid growth of most co-inoculated bacteria inhibited the growth and survival of the amoeba.

MeSH terms

  • Acanthamoeba / growth & development*
  • Animals
  • Antibiosis*
  • Coculture Techniques
  • Culture Media / metabolism
  • Escherichia coli / growth & development*
  • Pseudomonas aeruginosa / growth & development*
  • Serratia marcescens / growth & development*
  • Xanthomonas / growth & development*


  • Culture Media