Association of interleukin 6 release from endothelial cells and pulmonary hypertension in SLE

J Rheumatol. 1997 Mar;24(3):489-95.

Abstract

Objective: To investigate how sera from 37 patients with systemic lupus erythematosus (SLE) stimulate interleukin (IL) 6 release from IL-1beta pretreated endothelial cells and compare these effects to those of sera from 16 normal controls.

Methods: Endothelial cells pretreated 18 h with IL-1beta (5 U/ml) were incubated 2 h with sera diluted 10-fold with phosphate buffered saline (PBS). IL-6 concentrations in endothelial culture supernatants collected after incubation were measured by ELISA.

Results: Compared with PBS, sera from controls and 24 patients with SLE suppressed IL-6 release from IL-1beta pretreated cells. However, sera from 13 patients with SLE augmented IL-6 release. Of note, sera from 5 patients with pulmonary hypertension induced the highest level of IL-6 release. IgG from control sera suppressed IL-6 release, whereas F(ab')2 did not. Both IgG and F(ab')2 from the sera of patients with SLE with pulmonary hypertension augmented IL-6 release from IL-1beta pretreated cells.

Conclusion: IgG antiendothelial cell antibodies from patients with SLE may be associated with the pathogenesis of SLE and pulmonary hypertension.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Antibodies / blood
  • Endothelium, Vascular / cytology*
  • Endothelium, Vascular / immunology
  • Endothelium, Vascular / metabolism*
  • Female
  • Humans
  • Hypertension, Pulmonary / blood
  • Hypertension, Pulmonary / complications*
  • Immunoglobulin Fab Fragments / metabolism
  • Immunoglobulin M / immunology
  • Immunoglobulin M / metabolism
  • Interleukin-1 / pharmacology
  • Interleukin-6 / metabolism*
  • Lupus Erythematosus, Systemic / blood*
  • Lupus Erythematosus, Systemic / complications
  • Male
  • Middle Aged
  • Umbilical Veins

Substances

  • Antibodies
  • Immunoglobulin Fab Fragments
  • Immunoglobulin M
  • Interleukin-1
  • Interleukin-6