Identification of an epitope derived from human proteolipid protein that can induce autoreactive CD8+ cytotoxic T lymphocytes restricted by HLA-A3: evidence for cross-reactivity with an environmental microorganism

J Neuroimmunol. 1997 Mar;73(1-2):7-14. doi: 10.1016/s0165-5728(96)00161-0.


The demyelination process that occurs in the central nervous system (CNS) of patients with multiple sclerosis (MS) is in part due to an inflammatory response in which CD4+ and CD8+ T cells and macrophages infiltrate white matter. In this study, we have identified a peptide sequence derived from the CNS-specific myelin protein proteolipid protein (PLP) which could bind to HLA-A3 and induce a HLA-A3-restricted CD8+ CTL response from HLA-A3+ donors. These PLP peptide-specific CTL could lyse HLA-A3+ target cells pulsed with a homologous peptide derived from the CRM1 protein of Saccharomyces cerevisae. These findings demonstrate the immunogenic potential of a PLP-derived peptide for generation of autoreactive HLA-A3-restricted CD8+ CTL, and further show that these CTL can be activated by a peptide derived from a common environmental microorganism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Apoproteins / immunology*
  • Autoimmunity*
  • CD8-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / metabolism
  • Cell Line
  • Cross Reactions
  • Cytokines / metabolism
  • Epitopes*
  • Female
  • Fungal Proteins / immunology
  • HLA-A3 Antigen / immunology*
  • Humans
  • Karyopherins*
  • Male
  • Myelin Proteolipid Protein / immunology*
  • Peptide Fragments / immunology
  • Receptors, Cytoplasmic and Nuclear*
  • Saccharomyces cerevisiae / metabolism
  • T-Lymphocytes, Cytotoxic / immunology*


  • Apoproteins
  • Cytokines
  • Epitopes
  • Fungal Proteins
  • HLA-A3 Antigen
  • Karyopherins
  • Myelin Proteolipid Protein
  • Peptide Fragments
  • Receptors, Cytoplasmic and Nuclear
  • exportin 1 protein