Protection from experimental autoimmune encephalomyelitis (EAE): non-depleting anti-CD4 mAb treatment induces peripheral T-cell tolerance to MBP in PL/J mice

J Neuroimmunol. 1997 Mar;73(1-2):117-23. doi: 10.1016/s0165-5728(96)00188-9.


Following pre-treatment with a non-depleting anti-CD4 mAb (H129.19) that produces long-lasting receptor saturation, PL/J mice were fully protected from experimental auto-immune encephalomyelitis (EAE) induced by injection of myelin basic protein (MBP). These mice did not develop EAE following MBP re-challenge 5-10 weeks later when the CD4+ cells were no longer coated by the mAb and their lymph node cells were specifically unresponsive to MBP stimulation in vitro. Moreover, superantigen staphylococcal enterotoxin B (SEB) inoculation, which re-induces EAE in MBP immunized mice, failed to activate encephalitogenic T-cells in anti-CD4 + MBP treated mice, even after MBP re-challenge, indicating that tolerance in the peripheral T-cell compartment was achieved. However, MBP re-challenge 16 weeks later, but not SEB, produced an acute episode of EAE in these mice, while it failed to induce disease in a parallel group of adult thymectomized mice. These results indicate that no memory of the first priming exists at this time and that new MBP-specific T-cell precursors are peripheralized and produce EAE after MBP recognition.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • CD4 Antigens / immunology*
  • Disease Susceptibility
  • Drug Tolerance
  • Encephalomyelitis, Autoimmune, Experimental / immunology
  • Encephalomyelitis, Autoimmune, Experimental / prevention & control*
  • Enterotoxins / immunology
  • Female
  • Immune Tolerance
  • Mice
  • Mice, Inbred Strains
  • Myelin Basic Protein / immunology*
  • T-Lymphocytes / drug effects*
  • T-Lymphocytes / pathology
  • Thymectomy
  • Thymus Gland / cytology
  • Thymus Gland / immunology


  • Antibodies, Monoclonal
  • CD4 Antigens
  • Enterotoxins
  • Myelin Basic Protein
  • enterotoxin B, staphylococcal