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Comparative Study
. 1996;28(6):583-9.
doi: 10.3109/00365549609037965.

Borrelia Burgdorferi Sensu Lato Strains Isolated From Cutaneous Lyme Borreliosis Biopsies Differentiated by Pulsed-Field Gel Electrophoresis

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Comparative Study

Borrelia Burgdorferi Sensu Lato Strains Isolated From Cutaneous Lyme Borreliosis Biopsies Differentiated by Pulsed-Field Gel Electrophoresis

U Busch et al. Scand J Infect Dis. .

Abstract

25 skin biopsy isolates of Borrelia burgdorferi sensu lato, mainly from German patients with erythema migrans [EM], borrelial lymphocytoma [BL] and acrodermatitis chronica atrophicans [ACA], were species-differentiated using pulsed-field gel electrophoresis (PFGE). The isolates revealed 15 B. afzelii, 8 B. garinii and 2 B. burgdorferi sensu stricto species according to Miu I digestion. All 6 ACA isolates were identified as B. afzelii species, whereas the 2 borrelial lymphocytoma isolates were grouped into B. garinii species. 12 B. afzelii strains, including 4 ACA isolates, were further investigated by digestion with 5 restriction enzymes. Most of the strains revealed an individual pattern. No specific characteristically large restriction fragment pattern (LRFP) could be detected within the ACA and/or EM group. The ACA isolates could not be clearly differentiated from the EM isolates according to the LRFP patterns. PFGE is thus a highly effective method for detecting differences among B. afzelii isolates.

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