Characterization of a cartilage-derived 66-kDa protein (RGD-CAP/beta ig-h3) that binds to collagen

Biochim Biophys Acta. 1997 Mar 1;1355(3):303-14. doi: 10.1016/s0167-4889(96)00147-4.


A 66-kDa collagen fiber-associated protein (RGD-CAP) was isolated from a fiber-rich fraction of pig cartilage by ultrafiltration and collagen-affinity chromatography. Amino acid sequencing and cDNA cloning indicated that the RGD-CAP is identical or closely related to beta ig-h3 protein which is induced in human adenocarcinoma cells by transforming growth factor-beta (TGF-beta) (Skonier, J., Neubauer, M., Madisen, L., Bennett, K., Plowman, G.D., and Purchio, A.F. (1992) DNA Cell. Biol. 11, 511-522). The RGD-CAP, as well as beta ig-h3, has the RGD sequence in the C-terminal region. The native RGD-CAP bound to type I, II, and IV collagens even in the presence of 1 M NaCl. A recombinant preparation of RGD-CAP expressed in Escherichia coli cells also bound to collagen but not to gelatin. The RGD-CAP mRNA was expressed in chondrocytes throughout all stages, although the expression level was highest during the prehypertrophic stage. In addition, TGF-beta increased the RGD-CAP mRNA level in chondrocyte cultures. Since RGD-CAP transcripts were found in most tissues, this novel collagen-binding protein may play an important role in cell-collagen interactions in various tissues including developing cartilage.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Blotting, Southern
  • Cartilage / chemistry
  • Cartilage / cytology
  • Cartilage / metabolism*
  • Cell Differentiation / physiology
  • Cells, Cultured
  • Chromatography, Affinity
  • Cloning, Molecular
  • Collagen / metabolism*
  • DNA, Complementary / chemistry
  • Escherichia coli / genetics
  • Extracellular Matrix Proteins*
  • Gene Expression Regulation, Developmental
  • Molecular Sequence Data
  • Neoplasm Proteins / chemistry*
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / isolation & purification
  • Neoplasm Proteins / metabolism*
  • Protein Binding
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Restriction Mapping
  • Sequence Analysis
  • Sequence Homology, Amino Acid
  • Swine
  • Transforming Growth Factor beta*


  • DNA, Complementary
  • Extracellular Matrix Proteins
  • Neoplasm Proteins
  • Recombinant Proteins
  • Transforming Growth Factor beta
  • betaIG-H3 protein
  • Collagen

Associated data

  • GENBANK/D55717