Stimulation of splenocytes in C3H/HeJ mice with Porphyromonas gingivalis lipid A in comparison with enterobacterial lipid A

Immunobiology. 1996;196(4):399-414. doi: 10.1016/S0171-2985(96)80062-3.


Porphyromonas gingivalis 381 lipid A induced strong mitogenic response in splenic B cells separated from LPS-non-responsive C3H/HeJ mice as well as LPS-responsive C3H/HeN mice, by using a magnetic cell sorting system. The lipid A also exhibited mitogenic activity in splenic T cells. P. gingivalis lipid A induced lower production of interleukin-1 beta (IL-1 beta) in splenic macrophage cultures and exhibited a comparable IL-6 producing activity in splenic B cells of C3H/HeN mice as compared to Escherichia coli-type synthetic lipid A (compound 506) and monophosphoryl lipid A from Salmonella minnesota Re 595 (MLA). Furthermore, P. gingivalis lipid A, but not compound 506 nor MLA, induced low IL-1 beta and high IL-6 production in C3H/HeJ mice. C3H/HeJ T cells in response to P. gingivalis lipid A stimulation resulted in definite IL-2 mRNA and its production, whereas IL-4 response was scarcely active in both C3H/HeN and C3H/HeJ T cells. P. gingivalis lipid A increased tyrosine, serine/threonine phosphorylation of proteins with various major induced bands in splenic B cells of both C3H/HeN and C3H/HeJ mice. Thus, P. gingivalis lipid A stimulated the splenic immunocytes of C3H/HeJ as well as C3H/HeN mice.

Publication types

  • Comparative Study

MeSH terms

  • Adjuvants, Immunologic / pharmacology
  • Animals
  • B-Lymphocytes / drug effects
  • B-Lymphocytes / immunology
  • Escherichia coli / chemistry
  • Escherichia coli / immunology*
  • Lipid A / analogs & derivatives
  • Lipid A / immunology*
  • Lipid A / pharmacology*
  • Lymphocyte Activation / drug effects*
  • Macrophage Activation / drug effects
  • Macrophage Activation / immunology
  • Macrophages / drug effects
  • Macrophages / immunology
  • Male
  • Mice
  • Mice, Inbred C3H
  • Porphyromonas gingivalis / chemistry
  • Porphyromonas gingivalis / immunology*
  • Salmonella / chemistry
  • Salmonella / immunology*
  • Spleen / cytology
  • Spleen / drug effects
  • Spleen / immunology
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / immunology


  • Adjuvants, Immunologic
  • Lipid A
  • monophosphoryl lipid A