Inhibitory effects of caffeic acid ethyl ester on H2O2-induced cytotoxicity and DNA single-strand breaks in Chinese hamster V79 cells

Biosci Biotechnol Biochem. 1996 Feb;60(2):316-8. doi: 10.1271/bbb.60.316.

Abstract

Cytotoxicity and DNA single-strand breaks caused by H2O2 were assessed by a colony formation assay and a DNA precipitation assay, respectively, with Chinese hamster V79 cells. In both assays, caffeic acid ethyl ester showed protective effects. The structure-activity relationship showed that the o-dihydroxy structure of caffeic acid ethyl ester was essential for the protective effects.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caffeic Acids / pharmacology*
  • Cells, Cultured
  • Cricetinae
  • Cricetulus
  • DNA / drug effects
  • DNA / metabolism
  • DNA Damage*
  • Drug Interactions
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Hydrogen Peroxide / antagonists & inhibitors*
  • Hydrogen Peroxide / toxicity*
  • Structure-Activity Relationship

Substances

  • Caffeic Acids
  • ethyl ferulate
  • ethyl caffeate
  • DNA
  • Hydrogen Peroxide